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Description
Research Area
Epigenetics & Chromatin
Images & Validation
−Item 1 of 7
| Tested Applications | FC, IHC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; IHC: 1/100 - 1/500; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 7H4F8 |
| Immunogen | Purified recombinant fragment of human PCNA (AA: 53-196 ) expressed in E. Coli. |
| Target | Disease |
| Molecular Weight | 28.7kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of PCNA antibody
Flow cytometric analysis of MOLT4 cells lysates using PCNA antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded cervical cancer tissues using PCNA antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded colon cancer tissues using PCNA antibody
Western blot analysis of human PCNA using PCNA antibody
Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using PCNA antibody
Western blot analysis of A431 (Lane 1), HeLa (Lane 2), HepG2 (Lane 3), Raji (Lane 4), MOLT4 (Lane 5) cell lysate using PCNA antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
PCNA Antibody (orb153410)
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