PCNA Antibody (C-term) [Knockout Validated]

SKU: orb1927275

Description

Mouse Monoclonal Antibody (Mab)

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC - 1:2000, IHC-P - 1:25
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Hamster

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: B3379EV3X3X5
• Immunogen: This PCNA antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 236-261 amino acids from the C-terminal region of human PCNA. Antigen Region: 236-261 aa.
• Target: PCNA
• Molecular Weight: 28769 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA

All lanes: Anti-PCNA Antibody (C-term) at 1:1000 dilution (upper) Lane 1: Hela Lane 2: Hela-Knockout Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/8000 dilution. Predicted band size: 34 kDa

Immunohistochemical analysis of paraffin-embedded Human Ovarian cancer section using Pink1. Diluted at 1:2000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. lymph section using PCNA Antibody (C-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

All lanes: Anti-PCNA Antibody (C-term) at 1:2000 dilution + C2C12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 34KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PCNA Antibody (C-term) at 1:2000 dilution. Lane 1: C2C12 whole cell lysate. Lane 2: C6 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: L929 whole cell lysate. Lane 5: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 29 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining PCNA in Rat brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

PCNA

UniProt

P12004

RefSeq (Protein):NP_002583.1, NP_872590.1