PCK1 Antibody (C-term)

• Catalog Number: orb1928611
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB03646
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, WB: 1:2000, IHC-P-Leica: 1:250
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 69195 Da
• Target: This PCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 592-622 amino acids from the C-terminal region of human PCK1.
• UniProt ID: P35558
• NCBI: NP_002582.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The anti-PCK1 Pab is used in Western blot to detect PCK1 in mouse liver (Lane 1) and mouse small intestine tissue lysates (Lane 2).

Western blot analysis of PCK1 Antibody (C-term) in rat primary hepatocyte cell line lysates. PCK1 (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of PCK1 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Immunohistochemical analysis of paraffin-embedded human kidney tissue was performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-PCK1 Antibody (C-term) at 1:2000 dilution. Lane 1: Human liver lysate. Lane 2: Human kidney lysate. Lane 3: NCI-H460 whole cell lysate. Lane 4: Mouse liver lysate. Lane 5: Mouse kidney lysate. Lane 6: Rat kidney lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.