PAK1 Antibody

SKU: orb1253763

Description

PAK1 Antibody

Research Area

Cancer Biology, Cell Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: PAK1 (Phospho-Thr212) antibody was raised against a peptide sequence around phosphorylation site of threonine 212 (P-V-T (p) -P-T) derived from Human PAK1.
• Target: PAK1
• Molecular Weight: 68 kDa
• Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PAKalpha, C1orf215, LAP18, OP18, p21-activated kinase 1, PAK-1, p65-PAK, Alpha-PAK

Western blot analysis of lysed extracts from 293 cells untreated or treated with forskolin using PAK1 (Phospho-Thr212).

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1 (Phospho-Thr212). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using PAK1 (Phospho-Thr212).

Quick Database Links

Gene Symbol

PAK1

UniProt

Q13153

RefSeq (Protein):NP_001122092.1