p70 S6 kinase α (phospho Thr229) rabbit pAb

• Catalog Number: orb764265
• Category: Antibodies
• Description: Rabbit polyclonal antibody to p70 S6 kinase alpha (phospho-Thr229)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of Thr229. AA range:195-244
• MW: 60
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-RPS6KB1 antibody, anti-STK14A antibody, anti-
• Note: For research use only

Immunofluorescence analysis of Hela cell. 1, p70 S6 kinase α (phospho Thr229) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase-8 Monoclonal Antibody (2G12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western Blot analysis of various cells using Phospho-p70 S6 kinase α (T229) Polyclonal Antibody diluted at 1:500.

Western Blot analysis of LOVO cells using Phospho-p70 S6 kinase α (T229) Polyclonal Antibody diluted at 1:500.

Immunohistochemistry analysis of paraffin-embedded human brain, using p70 S6 Kinase (Phospho-Thr229) Antibody. The picture on the right is blocked with the phospho peptide.