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MAPK3 Antibody
SKU: orb1253726
Description
Research Area
Cancer Biology, Cell Biology
Images & Validation
−Item 1 of 2
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | p44/42 MAP Kinase (Phospho-Thr202) antibody was raised against a peptide sequence around phosphorylation site of threonine 202 (F-L-T (p) -E-Y) derived from Human p44/42 MAP Kinase. |
| Target | MAPK3 |
| Molecular Weight | 42, 44 kDa |
| Purification | Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Concentration | 1 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ERK1, ERT2, ERK-1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, p44-ERK1, p44-MAPK, PKD, PKD1, PRKCM, ERK, Extracellular signal- regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 1
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Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA for the indicated times, using p44/42 MAP Kinase (Phospho-Thr202).
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Thr202). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic, nuclear staining using p44/42 MAP Kinase (Phospho-Thr202).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001035145.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
MAPK3 Antibody (orb1253726)
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