P2RX4 Recombinant Mouse Monoclonal Antibody

SKU: orb1172844

Description

P2RX4 Recombinant Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: ICC, WB
• Dilution Range: WB=1:500-2000, ICC/IF=1:50-200
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: IgG2b/Kappa
• Clone No.: G6F3
• Target: P2RX4
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 4°C overnight, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: HeLa, SH-SY5Y, Rat brain, Mouse brain, Protein loading quantity: 20 µg, Exposure time: 660 s, Predicted MW: 43 kDa, Observed MW: 50 kDa.

Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:200, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172844.

Quick Database Links

Gene Symbol

P2RX4