Recombinant ODC1 Antibody / Ornithine Decarboxylase

SKU: orb639843

Description

Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Immunohistochemistry (FFPE): 0.25-0.5ug/ml,Western blot: 0.5-1ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant ODC1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: ODC1/3636R
• Immunogen: Recombinant human ODC-1 protein was used as the immunogen for this recombinant ODC1 antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human prostate carcinoma with recombinant ODC1 antibody (clone ODC1/3636R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human PC-3 cell lysate with recombinant ODC1 antibody. Predicted molecular weight ~51 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant ODC1 antibody (clone ODC1/3636R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P11926