Onjisaponin B

SKU: orb1297531

Description

Onjisaponin B is a research compound that activates autophagy through the AMPK-mTOR pathway, elevates NGF, and enhances clearance of mutant huntingtin and A53T α-synuclein. It is utilized in preclinical studies for neurodegenerative disease research, including Parkinson's, Alzheimer's, and Huntington's disease models.

Research Area

Cell Biology, Epigenetics & Chromatin, Neuroscience, Protein Biochemistry, Signal Transduction

Key Properties

• CAS Number: 35906-36-6
• MW: 1573.67
• Purity: 99.97% (May vary between batches)
• Formula: C₇₅H₁₁₂O₃₅
• SMILES: [H][C@@]1(O[C@H]2[C@H](C)O[C@@]([H])(O[C@H]3[C@H](OC(=O)[C@]45CCC(C)(C)C[C@@]4([H])C4=CC[C@]6([H])[C@@]7(C)C[C@H](O)[C@H](O[C@]8([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]8O)[C@@](C)(C(O)=O)[C@]7([H])CC[C@@]6(C)[C@]4(CO)CC5)O[C@H](C)[C@H](OC(=O)\C=C\c4ccc(OC)cc4)[C@@H]3O[C@]3([H])O[C@@H](C)[C@H](O)[C@@H](O)[C@H]3O)[C@H](O)[C@@H]2O)OC[C@@H](O[C@]2([H])O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O
• Target: Autophagy,Beta Amyloid,AMPK,mTOR,NF-κB,Apoptosis,Caspase
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (1.27 mM);DMSO:50 mg/mL (31.77 mM)

Bioactivity

• In Vitro:
  In the current study, we aimed to identify autophagy inducers from Chinese medicinal herbs as a potential neuroprotective agent that enhances the clearance of mutant huntingtin and α±-synuclein in PC-12 cells. Through intensive screening using the green fluorescent protein-light chain 3 (GFP-LC3) autophagy detection platform, we found that the ethanol extracts of Radix Polygalae (Yuan Zhi) were capable of inducing autophagy. Further investigation showed that among three single components derived from Radix Polygalae--i.e., polygalacic acid, senegenin and Onjisaponin B--Onjisaponin B was able to induce autophagy and accelerate both the removal of mutant huntingtin and A53T α±-synuclein, which are highly associated with Huntington disease and Parkinson disease, respectively. Our study further demonstrated that Onjisaponin B induces autophagy via the AMPK-mTOR signaling pathway

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AMPK, Autophagy, Inhibitor, Onjisaponin B, inhibit, mTOR, PGE

Compound Identifiers

PubChem CID

21669942