NRF1 Recombinant Mouse Monoclonal Antibody

• Catalog Number: orb1152052
• Category: Antibodies
• Description: NRF1 Recombinant Mouse Monoclonal Antibody
• Target: NRF1
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: IgG2b/Kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein G
• Immunogen: KLH conjugated synthetic peptide derived from human NRF1
• UniProt ID: Q16656
• MW: 55 kDa
• Tested applications: ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: erythroid derived 2; like 1; Locus control region
• Research Area: Cell Biology, DNA and RNA, DNA and RNA Research, E
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1: HeLa, 2: HEK-293, 3: Jurkat, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 54 kDa, Observed MW: 70 kDa.

Cell line: HepG2, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1152052.

Sample: Lane 1: Human Hela cell lysates, Lane 2: Human HepG2 cell lysates, Lane 3: Human SH-SY5Y cell lysates, Lane 4: Human 293T cell lysates, Lane 5: Human Jurkat cell lysates, Primary: Anti-NRF1 (orb1152052) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 55 kDa, Observed band size: 70 kDa.