NQO1 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb612235
• Category: Antibodies
• Description: NQO1 Recombinant Rabbit Monoclonal Antibody
• Target: NQO1
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: A synthesized peptide derived from human NQO1 (1-46/274aa)
• UniProt ID: P15559
• MW: 31 kDa
• Tested applications: FC, ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1:50-100
• Antibody Type: Primary Antibody
• Clone Number: 8T5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Azoreductase; Cytochrome b 5 reductase; DHQU; DIA
• Research Area: Cancer Research, Drug Metabolism, Metabolism
• Note: For research use only

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-NQO1 antibody (orb612235), dilution: 1:100, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1:1000. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: mouse kidney tissue lysate, Lane 2: SH-SY5Y cell lysate, Primary: Anti-NQO1 (orb612235) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 31 kD, Observed band size: 31 kD.