TTF-1 Antibody / NKX2.1

SKU: orb385837

Description

Thyroid transcription factor-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. TTF-1 reactivity is also seen in thyroid malignancies.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Reactivity: Human, Rat
• Application Notes:
  Titering of the TTF-1 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: HBNK2-1
• Immunogen: Recombinant protein was used as the immunogen for the TTF-1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human thyroid tissue with TTF-1 antibody (clone HBNK2-1). Required HIER: boil FFPE tissue sections in pH6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes.

IHC testing of FFPE human lung tissue with TTF-1 antibody (clone HBNK2-1). Required HIER: boil FFPE tissue sections in pH6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes.

IHC testing of FFPE rat lung tissue with TTF-1 antibody (clone HBNK2-1). Required HIER: boil FFPE tissue sections in pH6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes.

Quick Database Links

UniProt

P43699