NFκB-p105 (phospho Ser927) rabbit pAb

• Catalog Number: orb764386
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NF kappa B-p105 (phospho-Ser927)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human NF-kappaB p105/p50 around the phosphorylation site of Ser927. AA range:896-945
• MW: 110
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-NFKB1 antibody, anti-Nuclear factor NF-kappa-
• Note: For research use only

Western Blot analysis of various cells using Phospho-NFκB-p105 (S927) Polyclonal Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NF-kappaB p105/p50 (Phospho-Ser927) Antibody.

Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using NF-kappaB p105/p50 (Phospho-Ser927) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p105/p50 (Phospho-Ser927) Antibody. The picture on the right is blocked with the phospho peptide.