NF-L Mouse Monoclonal Antibody

SKU: orb1152078

Description

NF-L Mouse Monoclonal Antibody

Research Area

Cell Biology, Intermediate Filaments, Neuroscience, Stem Cell Research, neurofilaments

Images & Validation

• Tested Applications: ELISA
• Dilution range: ELISA=1:5000-10000
• Reactivity: Mouse, Rat
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 6F2
• Immunogen: Recombinant Human NF-L Protein (1-543/543)
• Target: NEFL
• Molecular Weight: 68 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS (pH7.4).
• Disclaimer: For research use only

Alternative Names

Neurofilament L; Neurofilament 68; Neurofilament triplet L; 70 kD Neurofilament Light; 68kDa neurofilament protein; CMT 1F; CMT 2E; CMT1F; CMT2E; FLJ53642; Light molecular weight neurofilament protein; NEFL; Neurofilament light; Neurofilament light polypeptide 68kDa; Neurofilament light polypeptide; Neurofilament protein, light chain; Neurofilament subunit NF L; Neurofilament triplet L protein; NF 68; NF L; NF68; NFL; NFL_HUMAN.

Measured by its binding ability in a indirect ELISA. Immobilized Human NF-L protein, His Tag at 2 µg/mL (100 µl/well) can bind Mouse Anti-Human NF-L Antibody, the EC50 is 16.47 ng/ml.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (orb1152078) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (orb1152078) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (orb1152078) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (orb1152078) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Recombinant Human NF-L Protein at 100 ng, Primary: Mouse Anti-NF-L Monoclonal Antibody at 1/1000 dilution (Cat.orb1152078), Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 61.4kD, Observed band size: 68/44kD.

Quick Database Links

Gene Symbol

NEFL

UniProt

P07196