RBFOX3 Antibody

SKU: orb1274564

Description

RBFOX3 Antibody

Research Area

Epigenetics & Chromatin, Neuroscience

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NeuN (NP_001076044.1).
• Target: RBFOX3
• Molecular Weight: Observed: 50kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

FOX-3, FOX3, HRNBP3, NEUN, RNA binding protein fox-1 homolog 3, RNA binding protein, fox-1 homolog 3, fox-1 homolog C, hexaribonucleotide binding protein 3, neuN antigen, neuronal nuclei antigen

Western blot analysis of extracts of various cell lines, using NeuN antibody at 1:1000 dilution.

Western blot analysis of extracts from normal (control) and NeuN knockout (KO) 293T cells, using NeuN antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded rat brain using NeuN antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using NeuN antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using NeuN antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-251MG cells using NeuN antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

RBFOX3

UniProt

A6NFN3