NEDD4L Antibody

SKU: orb1256003

Description

NEDD4L Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 836-955 of human NEDD4L (NP_056092.2).
• Target: NEDD4L
• Molecular Weight: Observed: 113kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

E3 ubiquitin-protein ligase NEDD4-like, 632-, NEDD42, Nedd4-2, NEDD4L, KIAA0439, NEDL3

Western blot analysis of extracts of various cell lines, using NEDD4L antibody (orb1256003) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat kidney using NEDD4L antibody (orb1256003) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human prostate using NEDD4L antibody (orb1256003) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of U2OS cells using NEDD4L antibody (orb1256003) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

NEDD4L

UniProt

Q96PU5