NCAM / CD56 Antibody

• Catalog Number: orb248478
• Category: Antibodies
• Description: This antibody reacts with an extracellular domain (close to transmembrane) of NCAM (Neural cell adhesion molecule), also called CD56. Three isoforms of NCAM / CD56 are produced by differential splicing of the RNA transcript from a single gene. The 135 kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. NCAM antibody recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). NCAM antibody plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 123C3.D5
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: A membrane preparation of a small cell lung carcinoma was used as the immunogen for this CD56 / NCAM antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This NCAM antibody is available for research use only.
• Entrez: 4684
• Storage: Store the NCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of lymphocyte gated human PBM cells using NCAM antibody (clone 123C3.D5, orange) and unstained cells (gray).

IHC testing of FFPE human colon ganglion tissue stained with NCAM antibody (clone 123C3.D5).

SDS-PAGE Analysis of Purified, BSA-Free NCAM Antibody (clone 123C3.D5). Confirmation of Integrity and Purity of the Antibody.