Multi-rAb CoraLite Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L)

SKU: orb2302682

Description

This Multi-rAb antibody targets Rabbit IgG (H+L) in FC and IF. It is higly specific to rabbit IgG and shows minimal reactivity for mouse, rat, human, pig and bovine IgGs.

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution range: 1:200-1:1000 IF and for FC, 1:5000-1:20,000 for western blotting
• Reactivity: Mouse

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Goat
• Clonality: Recombinant
• Purity: The antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads.
• Conjugation: CoraLite® Plus 750

Storage & Handling

• Storage: Store at -20°C. Stable for one year after shipment.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 50% glycerol, 10 mg/mL rAlbumin, 0.1% Proclin-300, pH 7.4.
• Concentration: 0.5 mg/mL
• Disclaimer: For research use only

Various lysates were subjected to SDS-PAGE followed by western blot with Rabbit anti-Beta Tubulin recombinant antibody. Multi-rAb CoraLite Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) orb2302682 was used at 1:10000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with Rabbit anti-Beta Catenin recombinant antibody at dilution of 1:40000. Multi-rAb CoraLite Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) orb2302682 was used at 1:10000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with Rabbit anti-Beta Tubulin recombinant antibody at dilution of 1:20000. Multi-rAb CoraLite Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) orb2302682 was used at 1:10000 for detection.

Immunofluorescent analysis of at dilution of 1:200 and Multi-rAb CoraLite Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302682), Beta Actin antibody ( Clone: 2D4H5).

Immunofluorescent analysis of at dilution of 1:200 and Multi-rAb CoraLite Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302682), CL488-Phalloidin (green).

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-HLA class I rabbit polyclonal antibody and Rabbit IgG Isotype Control. Multi-rAb CoraLite Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) orb2302682 was used at 1:500 for detection.