Multi-rAb CoraLite Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L)

SKU: orb2302684

Description

This Multi-rAb antibody targets Rabbit IgG (H+L) in FC and IF. It is higly specific to rabbit IgG and shows minimal reactivity for mouse, rat, human, pig and bovine IgGs.

Images & Validation

• Tested Applications: FC, IF
• Dilution range: 1:200-1:1000 for IF and FC
• Reactivity: Rabbit

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Goat
• Clonality: Recombinant
• Purity: The antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads.

Storage & Handling

• Storage: Store at -20°C. Stable for one year after shipment.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 50% glycerol, 10 mg/mL rAlbumin, 0.1% Proclin-300, pH 7.4.
• Concentration: 0.5 mg/mL
• Disclaimer: For research use only

Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (1:2000, red) and mouse anti-Alpha Tubulin monoclonal antibody (1:1000, green). Multi-rAb CoraLite Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302684, 1:500) and Multi-rAb CoraLite Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (orb2302693, 1:500) were used for detection.

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (1:200, red) and mouse anti-NPM1 monoclonal antibody (1:1000, green). Multi-rAb CoraLite Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302684, 1:500) and Multi-rAb CoraLite Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (orb2302693, 1:500) were used for detection.

Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (1:200, red) and mouse anti-Calbindin-D28k monoclonal antibody (1:200, green). Multi-rAb CoraLite Plus 594 conjugated Recombinant Goat anti-rabbit secondary antibody (orb2302684, 1:500) and Multi-rAb CoraLite Plus 488 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (orb2302693, 1:500).

Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (1:200, red) and mouse anti-NeuN monoclonal antibody (green). Multi-rAb CoraLite Plus 594 conjugated Recombinant Goat anti-rabbit secondary antibody (orb2302684, 1:500) and Multi-rAb CoraLite Plus 488 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (orb2302693, 1:500).

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse small intestine tissue using smooth muscle actin antibody at dilution of 1:400 and Multi-rAb CoraLite Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302684).

Immunofluorescent analysis of at dilution of 1:400 and Multi-rAb CoraLite Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302684).

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Band 3 / AE1 antibody (Clone: 3K21 ) at dilution of 1:200 and Multi-rAb CoraLite Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302684). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-HLA class I rabbit polyclonal antibody and Rabbit IgG Isotype Control. Multi-rAb CoraLite Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) orb2302684 was used at 1:500 for detection.