MUC5AC Antibody

SKU: orb1252729

Description

MUC5AC Antibody

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC
• Reactivity: Feline, Gallus, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 µg/mLIHC (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC5AC antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: 45M1
• Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for this MUC5AC antibody.
• Target: MUC5AC
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MUC5AC, Gastric mucin, Major airway glycoprotein, Muc5/5ac, Mucin-5AC, LeB, Lewis B blood group antigen, Tracheobronchial mucin, TBM, MUC-5AC, MUC5

IHC testing of human stomach stained with MUC5AC antibody (45M1).

Intracellular flow cytometry staining of A549 cells using MUC5AC antibody (red) and isotype control (green).

IHC testing of human gastric carcinoma stained with MUC5AC antibody (45M1).

IHC testing of rat stomach stained with MUC5AC antibody (45M1).

Quick Database Links

Gene Symbol

MUC5AC

UniProt

P98088