MSH2 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1152003
• Category: Antibodies
• Description: MSH2 Recombinant Rabbit Monoclonal Antibody
• Target: MSH2
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: A synthesized peptide derived from human MSH2 (1-50/934aa)
• UniProt ID: P43246
• MW: 105 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:1000-5000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000, Flow-Cyt=1ug/Test
• Antibody Type: Primary Antibody
• Clone Number: B4F3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MSH-2; BAT26; DNA mismatch repair protein Msh2; FC
• Research Area: Cancer Research, Tumor Suppressors
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Lysate: HeLa, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 105 kDa, Observed MW: 104 kDa.

Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilisation: 0.1% TritonX-100, Primary ab Dilution: 1:100, Primary incubation condition: 1 hour at room temperature, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152003 (green).

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human SW480 cell lysates, Lane 3: Human K562 cell lysates, Primary: Anti-MSH2 (orb1152003) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 105 kDa, Observed band size: 108 kDa.