Mouse Protein Z Dependent Protease Inhibitor (ZPI) ELISA Kit

SKU: orb780007

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Z Dependent Protease Inhibitor(ZPI).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 60 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ZPI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ZPI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ZPI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ZPI in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.94-60 ng/mL
• Sensitivity: 0.37 ng/mL
• Concentration: 60 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

PZI ELISA Kit, SERPINA10 ELISA Kit, Serine(Cysteine)Proteinase Inhibitor,Clade A(Alpha-1 Antiproteinase,Antitrypsin)Member 10 ELISA Kit, Protein Z-Related Protease Inhibitor ELISA Kit

Quick Database Links

UniProt

Q8R121