MLH1 Antibody / MutL Homolog 1

• Catalog Number: orb1151494
• Category: Antibodies
• Description: Rabbit monoclonal antibody to MLH1
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length human MLH1 protein was used as the immunogen for the recombinant MLH1 antibody.
• UniProt ID: P40692
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the recombinant MLH1 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: MLH1/6284R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Cancer, DNA Damage, Epigenetics
• Note: For research use only

IHC staining of FFPE human colon carcinoma tissue with recombinant MLH1 antibody (clone MLH1/6284R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE Lynch Syndrome / Hereditary Non-Polyposis Colorectal Cancer (HNPCC) with recombinant MLH1 antibody (clone MLH1/6284R) at 2 ug/ml in PBS, 30 min RT. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human Hereditary Nonpolyposis Colorectal Cancer (HNPCC) with recombinant MLH1 antibody (MLH1/6284R) at 2 ug/ml.