Recombinant MCM7 Antibody

SKU: orb606967

Description

MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Titering of the recombinant MCM7 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: MCM7/2832R
• Immunogen: A human recombinant partial protein corresponding to amino acids 195-319 was used as the immunogen for the recombinant MCM7 antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of FFPE human tonsil with recombinant MCM7 antibody. Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

IHC testing of FFPE human lymph node with recombinant MCM7 antibody. Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

Western blot testing of human 1) MCF7, 2) HEK293, 3) A549, 4) SKBR3 and 5) HeP2 cell lysate with MCM7 antibody (clone MCM7/2832R). Expected molecular weight: 80~90 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant MCM7 antibody (clone MCM7/2832R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P33993