Mad2L1 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb704306
• Category: Antibodies
• Description: Mad2L1 Recombinant Rabbit Monoclonal Antibody
• Target: MAD2L1
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: Recombinant human Mad2L1 protein
• UniProt ID: Q13257
• MW: 23 kDa
• Tested applications: ICC
• Dilution range: ICC/IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: 6C6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HSMAD2; Human mitotic feedback control protein Mad
• Research Area: Cancer Research, Cell Biology, Cell Cycle, Immunol
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Mad2L1) monoclonal Antibody, Unconjugated (orb704306) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

ICC staining Mad2L1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Western blot analysis of Mad2L1 on different cell lysates using anti-Mad2L1 antibody at 1/500 dilution. Positive control: Lane 1: K562, Lane 2: 293T, Lane 3: SH-SY5Y.