LRP1 Antibody: Biotin

• Catalog Number: orb535885
• Category: Antibodies
• Description: Rabbit polyclonal antibody against LRP-1 conjugated to Biotin
• Target: LRP-1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Biotin
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Immunogen: Synthetic peptide from the N-terminal of Human Low-density lipoprotein receptor-related protein 1 (aa. 160-260)
• UniProt ID: Q07954
• MW: 80 kDa
• Tested applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:1000); IHC (1:50)
• Application notes: A 1:1000 dilution of SPC-785 was sufficient for detection of LRP-1 in 15 µg of mouse Brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: LRPICD antibody, APOER antibody, LRP-85 antibody,
• Note: For research use only
• Entrez: 4035
• NCBI: NP_002323.2
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Rabbit Anti-LRP1 Polyclonal Antibody. Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-LRP1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Western blot analysis of Mouse, Rat Brain showing detection of 504.6 kDa LRP-1 protein using Rabbit Anti-LRP-1 Polyclonal Antibody. Load: 15 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-LRP-1 Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 504.6 kDa. Other Band (s): 80 kDa.