LC3 Antibody

SKU: orb97657

Description

Mouse monoclonal antibody to LC3

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research, Microbiology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:8000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1 k
• Clone No.: 166AT1234
• Immunogen: This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).
• Target: This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ATG8F antibody, anti Autophagy-related protein LC3 B antibody, anti Autophagy-related ubiquitin-like modifier LC3 B antibody, anti LC3 antibody, anti LC 3 antibody, anti LC3A antibody, anti LC3B antibody, anti LC3II antibody, anti MAP1 light chain 3 like protein 2 antibody, anti MAP1 light chain 3-like protein 2 antibody, anti MAP1A/1BLC3 antibody, anti MAP1A/MAP1B LC3 B antibody, anti MAP1A/MAP1B light chain 3 B antibody, anti MAP1ALC3 antibody, anti MAP1LC3B a antibody, anti Map1lc3b antibody, anti Microtubule associated protein 1 light chain 3 beta antibody, anti Microtubule-associated protein 1 light chain 3 beta antibody, anti Microtubule-associated proteins 1A/1B light chain 3B antibody

All lanes: Anti-MAP1LC3A Antibody at dilution. Lane 1: Mouse brain whole cell lysate. Lane 2: Rat brain whole cell lysate. Lane 3: Human brain whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 14 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).

UniProt

Q9GZQ8, Q9H492

RefSeq (Protein):NP_852610.1, NP_115903.1

Graham, Christopher D. et al. Tamoxifen Induces Cytotoxic Autophagy in Glioblastoma J Neuropathol Exp Neurol, 75, 946-954 (2016)

PubMed: 27516117