LAMP2 Antibody

SKU: orb1939405

Description

Mouse Monoclonal Antibody (Mab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: IF - 1:10-50, IHC - 1:500, WB - 1:2000-8000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,K
• Clone No.: B379EV38X8X8
• Immunogen: This LAMP2 monoclonal antibody is generated from mouse immunized with LAMP2 recombinant protein.
• Target: LAMP2
• Molecular Weight: 44961 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lysosome-associated membrane glycoprotein 2, LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, CD107b, LAMP2

All lanes: Anti-LAMP2 Antibody at 1:2000-8000 dilution. Lane 1: HeLat whole cell lysate. Lane 2: T47D whole cell lysate. Lane 3: Jurkat whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of LAMP2 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunohistochemical analysis of paraffin-embedded Human lung section using Pink1. diluted at 1:500 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Quick Database Links

Gene Symbol

LAMP2

UniProt

P13473

RefSeq (Protein):NP_054701.1, NP_002285.1, NP_001116078.1