IKB alpha Mouse Monoclonal Antibody

SKU: orb526702

Description

IKB alpha Mouse Monoclonal Antibody

Research Area

NF-kB Signalling Pathway, TLR Signaling Pathway

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ICC/IF=1:50-200
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 2A5
• Immunogen: Recombinant human IKB alpha Protein (1-77/314aa)
• Target: NFKBIA
• Molecular Weight: 36 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NFKBIA; Inhibitor of KB alpha; I kappa B alpha; I(Kappa)B(alpha); IkappaBalpha; IKBA; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer binding protein MAD3; NF kappa B inhibitor alpha; NFKBI; NFKBIA; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha; IKBA_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IKB alpha) Monoclonal Antibody, Unconjugated (orb526702) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-IKB alpha (orb526702) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 36 kD.

Sample: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-IKB alpha (orb526702) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 36 kD.

Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Lane 3: Siha (Human) Cell Lysate at 30 ug, Primary: Anti-IKB alpha (orb526702) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 39 kD, Observed band size: 39 kD.

Quick Database Links

Gene Symbol

NFKBIA

UniProt

P25963