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Human UBE2K protein

Human UBE2K protein

Catalog Number: orb392663

DispatchUsually dispatched within 5-10 working days
$ 100.00
Catalog Numberorb392663
CategoryProteins
DescriptionRecombinant human UBE2K protein is produced by our E.coli expression system and the target gene encoding Met1-Asn200 is expressed with a GST tag at the N-terminus.
TargetUBE2K
ReactivityHuman
Form/AppearanceLiquid in a 0.2 μM filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.
Buffer/PreservativesLiquid in a 0.2 μM filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.
PurityGreater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Protein SequenceMSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMANI AVQRIKREFK EVLKSEETSK NQIKVDLVDE NFTELRGEIA GPPDTPYEGG RYQLEIKIPE TYPFNPPKVR FITKIWHPNI SSVTGAICLD ILKDQWAAAM TLRTVLLSLQ ALLAAAEPDD PQDAVVANQY KQNPEMFKQT ARLWAHVYAG APVSSPEYTK KIENLCAMGF DRNAVIVALS SKSWDVETAT ELLLSN
UniProt IDP61086
Tested applicationsELISA, MS, SDS-PAGE, WB
Application notesAlways centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
EndotoxinsEndotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
SourceE. coli
StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Alternative namesHIP2; LIG; Ubiquitin-conjugating enzyme E2 K; Hunt
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NoteFor research use only
Entrez3093
  • Human UBE2K protein [orb245434]

    Greater than 90% as determined by SDS-PAGE.

    26.3 kDa

    E.coli

    20 μg, 100 μg, 1 mg
  • Human Huntingtin Interacting Protein 2 (HIP2) ELISA Kit [orb778869]

    Human

    0.16-10 ng/mL

    0.056 ng/mL

    96 T, 48 T
  • UBE2K siRNA (Human) [orb1866151]

    RNA-I

    30 nmol, 15 nmol
  • Human UBE2K protein [orb392664]

    ELISA,  MS,  SDS-PAGE,  WB

    Greater than 95% as determined by reducing SDS-PAGE.

    E. coli

    10 μg, 50 μg, 500 μg
  • Human UBC1 protein [orb107811]

    HPLC,  SDS-PAGE

    Unconjugated

    > 95% by SDS-PAGE and HPLC analyses

    23.4 kDa

    10 μg, 100 μg, 500 μg