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Catalog Number | orb392661 |
---|---|
Category | Proteins |
Description | Recombinant human UBE2I protein is produced by our E. coli expression system and the target gene encoding Met1-Ser158 is expressed with a GST tag at the N-terminus. |
Target | UBE2I |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 50mM HEPES, 150mM NaCl, pH 7.5. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 50mM HEPES, 150mM NaCl, pH 7.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Protein Sequence | MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMSG IALSRLAQER KAWRKDHPFG FVAVPTKNPD GTMNLMNWEC AIPGKKGTPW EGGLFKLRML FKDDYPSSPP KCKFEPPLFH PNVYPSGTVC LSILEEDKDW RPAITIKQIL LGIQELLNEP NIQDPAQAEA YTIYCQNRVE YEKRVRAQAK KFAPS |
UniProt ID | P63279 |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Source | E. coli |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Alternative names | UBC9; UBCE9; SUMO-conjugating enzyme UBC9; SUMO-pr Read more... |
Note | For research use only |
Entrez | 7329 |
Greater than 90% as determined by SDS-PAGE. | |
44.9 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
37.7 kDa | |
E.coli |
FC, ICC, IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
Human | |
0.16-10 ng/mL | |
0.059 ng/mL |