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Catalog Number | orb392660 |
---|---|
Category | Proteins |
Description | Recombinant human UBE2H protein is produced by our E.coli expression system and the target gene encoding Met1-Leu183 is expressed with a GST tag at the N-terminus. |
Target | UBE2H |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Protein Sequence | MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMSS PSPGKRRMDT DVVKLIESKH EVTILGGLNE FVVKFYGPQG TPYEGGVWKV RVDLPDKYPF KSPSIGFMNK IFHPNIDEAS GTVCLDVINQ TWTALYDLTN IFESFLPQLL AYPNPIDPLN GDAAAMYLHR PEEYKQKIKE YIQKYATEEA LKEQEEGTGD SSSESSMSDF SEDEAQDMEL |
UniProt ID | P62256 |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Source | E. coli |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Alternative names | Ubiquitin-conjugating enzyme E2 H; UbcH2; Ubiquiti Read more... |
Note | For research use only |
Entrez | 7328 |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
20 kDa | |
E.Coli |
> 93%, determined by SDS-PAGE | |
This protein contains the human UBE2H (P62256) (Met 1-Leu 183) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus and expressed from E. coli. |
Greater than 90.0% as determined by SDS-PAGE. | |
Escherichia Coli |