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Item 1 of 4
Item 1 of 4
Human SIRPa Protein, hFc-His Tag
Catalog Number: orb689370
| Catalog Number | orb689370 |
|---|---|
| Category | Proteins |
| Description | Recombinant Human SIRPa with C-terminal human Fc and 6×His tag |
| Reactivity | Human |
| Tag | C-Human Fc and 6×His Tag |
| Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| MW | The protein has a predicted molecular mass of 70-98 kDa after removal of the signal peptide. |
| Target | SIRPa |
| UniProt ID | P78324 |
| Source | Mammalian |
| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| Alternative names | SHPS1;SIRPA;CD172A;BIT;MFR;MYD1;P84;PTPNS Read more... |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
Human SIRPα, hFc-His Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD47, mFc-His tagged protein orb689369 can bind its native ligand Human SIRPα, hFc-His tagged protein (orb689370) in a linear range of 3.3-26.37 ng/ml.
A: Human SIRPα, hFc-His tagged protein without freeze-thaw treatment. B: Human SIRPα, hFc-His tagged protein after one freeze-thaw cycle. C: Human SIRPα, hFc-His tagged protein after three freeze-thaw cycles. D: Human SIRPα, hFc-His tagged protein after five freeze-thaw cycles.
Lane 1: Human SIRPα, hFc-His tagged protein without freeze-thaw treatment, Lane 2: Human SIRPα, hFc-His tagged protein after one freeze-thaw cycle, Lane 3: Human SIRPα, hFc-His tagged protein after three freeze-thaw cycles, Lane 4: Human SIRPα, hFc-His tagged protein after five freeze-thaw cycles.
