Human Sequestosome-1 (SQSTM1) ELISA Kit

SKU: orb3146079

Description

This Human Sequestosome-1 (SQSTM1) ELISA Kit employs a two-site sandwich ELISA to quantitate SQSTM1.

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  This Human Sequestosome-1 (SQSTM1) ELISA Kit employs a two-site sandwich ELISA to quantitate SQSTM1 in samples. An antibody specific for SQSTM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySQSTM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SQSTM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SQSTM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Key Properties

• Reactivity: Human
• Sample Types: Cell culture supernatants#Serum#Plasma#Other biological fluids
• Range: Please inquire
• Sensitivity: Please inquire

Storage & Handling

• Storage: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
• Expiration Date: 6 months from date of receipt.
• Hazard Information: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
• Disclaimer: For research use only

Alternative Names

SQSTM1; A170; OSIL; PDB3; ZIP3; p60; p62; p62B; EBI3-associated protein p60; oxidative stress induced like; phosphotyrosine independent ligand for the Lck SH2 domain p62; ubiquitin-binding protein p62