Human Receptor II for the Fc Region of Immunoglobulin G (FcgRII) ELISA Kit

SKU: orb775935

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor II For The Fc Region Of Immunoglobulin G(FcgRII).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 3000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FcgRII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FcgRII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FcgRII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FcgRII in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 46.88-3000 pg/mL
• Sensitivity: 16.58 pg/mL
• Concentration: 3000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD32 ELISA Kit, FCGR2A ELISA Kit, FCG2 ELISA Kit, FCGR2A1 ELISA Kit, FCGR2 ELISA Kit, FCGR2B ELISA Kit, FCGR2C ELISA Kit, Fc Fragment Of IgG, Low Affinity IIa, Receptor ELISA Kit, Low affinity immunoglobulin gamma Fc region receptor II-a ELISA Kit