Human Prostaglandin F2 Alpha (PGF2a) ELISA Kit

• Catalog Number: orb2668220
• Category: Assays and Kits
• Description: Human Prostaglandin F2 Alpha (PGF2a) ELISA Kit
• Application notes: standard: 800 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PGF2α protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 12.5-800 pg/mL
• Sensitivity: 3.65 pg/mL
• Sample Types: Serum, plasma, saliva and other biological fluids.
• Alternative names: 8-isoprostane; 8-iso-PGF2α
• Note: For research use only