Human Peroxiredoxin 6 (PRDX6) ELISA Kit

SKU: orb777999

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxiredoxin 6(PRDX6).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRDX6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PRDX6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PRDX6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRDX6 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.121 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

1-Cys ELISA Kit, AOP2 ELISA Kit, NSGPx ELISA Kit, PRX ELISA Kit, aiPLA2 ELISA Kit, p29 ELISA Kit, Acidic calcium-independent phospholipase A2 ELISA Kit, Antioxidant protein 2 ELISA Kit, Non-selenium glutathione peroxidase ELISA Kit, Red blood cells page spot 12 ELISA Kit

Quick Database Links

UniProt

P30041