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Human Oxidized Low Density Lipoprotein (OxLDL) ELISA Kit

Catalog Number: orb779265

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb779265
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Oxidized Low Density Lipoprotein(OxLDL) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Oxidized Low Density Lipoprotein(OxLDL).
ReactivityHuman
Concentration100 ng/mL
Tested applicationsELISA
Application notesstandard: 100 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human OxLDL protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OxLDL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OxLDL in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeCompetitive
Assay Time2.5h
Range1.57-100 ng/mL
Sensitivity0.51 ng/mL
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Alternative namesOL ELISA Kit
NoteFor research use only
Human Oxidized Low Density Lipoprotein (OxLDL) ELISA Kit

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