Human N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) EasyStep ELISA Kit

• Catalog Number: orb1950025
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP, and the Human NT-ProBNP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human NT-ProBNP. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Concentration: 60 ng/mL
• Application notes: standard: 60 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP, and the Human NT-ProBNP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human NT-ProBNP. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 1.5h
• Range: 0.94-60 ng/mL
• Sensitivity: 0.94 ng/mL
• Sample Types: Serum, plasma
• Alternative names: NT-Pro-BNP; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29
• Research Area: Cardiovascular Research, Metabolism Research
• Note: For research use only
• Expiration Date: Please enquire.