Human N-MIDOsteocalcin (N-MID-OT) ELISA Kit

• Catalog Number: orb1146745
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-MIDOsteocalcin(N-MID-OT). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to N-MIDOsteocalcin(N-MID-OT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-MIDOsteocalcin(N-MID-OT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-MIDOsteocalcin(N-MID-OT) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Concentration: 40 ng/mL
• Application notes: standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human N-MID-OT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human N-MID-OT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human N-MID-OT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human N-MID-OT in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.63-40 ng/mL
• Sensitivity: 0.39 ng/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: N-MID-OT
• Research Area: Metabolism Research, Musculoskeletal & Connective
• Note: For research use only