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| Catalog Number | orb1950182 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MPO, and the Human MPO standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MPO. After TMB substrate solution is added, only those wells that contain Human MPO and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MPO in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Range | 0.63-40 ng/mL |
| Assay Time | 1.5h |
| Concentration | 40 ng/mL |
| UniProt ID | P05164 |
| Sensitivity | 184 pg/mL |
| Alternative names | Mpo; Myeloperoxidase; MPO Read more... |
| Note | For research use only |
| Application notes | standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MPO, and the Human MPO standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MPO. After TMB substrate solution is added, only those wells that contain Human MPO and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MPO in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma |
| Expiration Date | Please enquire. |
