Human Leukocyte Elastase Inhibitor (LEI) ELISA Kit

SKU: orb778071

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukocyte Elastase Inhibitor(LEI).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LEI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LEI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LEI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LEI in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.121 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

SERPINB1 ELISA Kit, EI ELISA Kit, ELANH2 ELISA Kit, LEI ELISA Kit, M/NEI ELISA Kit, MNEI ELISA Kit, PI2 ELISA Kit, Serpin Peptidase Inhibitor Clade B Member 1 ELISA Kit, Anti-Elastase ELISA Kit, Monocyte/Neutrophil Elastase Inhibitor ELISA Kit

Quick Database Links

UniProt

P30740