Human IL-12p40(Interleukin 12 p40) Microsample ELISA Kit

• Catalog Number: orb2998860
• Category: Assays and Kits
• Description: Human IL-12p40(Interleukin 12 p40) Microsample ELISA Kit
• UniProt ID: P29460
• Application notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 7.82-500 pg/mL
• Sensitivity: 2.9 pg/mL
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Alternative names: IL-12B; p40; IL12B; CLMF2; NKSF2; Natural Killer C
• Note: For research use only