Human Hemoglobin Beta (HBb) ELISA Kit

• Catalog Number: orb777935
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hemoglobin Beta(HBb) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hemoglobin Beta(HBb).
• Reactivity: Human
• Concentration: 500 pg/mL
• UniProt ID: P68871
• Tested applications: ELISA
• Application notes: standard: 500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBb in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 7.82-500 pg/mL
• Sensitivity: 2.82 pg/mL
• Sample Types: serum, plasma and erythrocyte lysates
• Alternative names: HB-B ELISA Kit, HBD ELISA Kit, CD113t-C ELISA Kit,
• Research Area: Hematology
• Note: For research use only
• Expiration Date: Please enquire.