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Catalog Number | orb705312 |
---|---|
Category | Proteins |
Description | Recombinant Human Glutathione S-transferase kappa 1(GSTK1) (Active) |
Tag | N-terminal GST-tagged |
Form/Appearance | Liquid or Lyophilized powder |
Buffer/Preservatives | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Protein Sequence | GPLPRTVELFYDVLSPYSWLGFEILCRYQNIWNINLQLRPSLITGIMKDSGNKPPGLLPRKGLYMANDLKLLRHHLQIPIHFPKDFLSVMLEKGSLSAMRFLTAVNLEHPEMLEKASRELWMRVWSRNEDITEPQSILAAAEKAGMSAEQAQGLLEKIATPKVKNQLKETTEAACRYGAFGLPITVAHVDGQTHMLFGSDRMELLAHLLGEKWMGPIPPAVNARL |
Protein Length | Partial |
UniProt ID | Q9Y2Q3 |
MW | 52.4 kDa |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Endotoxins | Not test. |
Source | E.coli |
Biological Origin | Homo sapiens (Human) |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized HTR1B at 5 μg/ml can bind human GSTK1,the EC50 of human GSTK1 protein is 159.40-218.50 ng/ml. |
Expression Region | 2-226aa |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Alternative names | GST 13-13 GST class-kappa GSTK1-1 |
Note | For research use only |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Measured by its binding ability in a functional ELISA. Immobilized HTR1B at 5 μg/ml can bind human GSTK1, the EC50 of human GSTK1 protein is 159.40-218.50 ng/ml.
> 90% as determined by SDS-PAGE. | |
26.7 kDa |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
24.6 kDa | |
E.Coli |
> 95%, determined by SDS-PAGE | |
This protein contains the mature form of human GSTK1 (Q9Y2Q3-1) (Gly2-Leu226) was expressed with a N-terminal Met and expressed from E. coli. |
ELISA, WB | |
Greater than 90% by SDS-PAGE gel analyses | |
46.1 KDa | |
E.Coli |
IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |