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Catalog Number | orb545735 |
---|---|
Category | Proteins |
Description | Human GM-CSF R alpha, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 320 (Accession # P15509-1). |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 60.9 kDa |
Target | GM-CSF R alpha |
UniProt ID | P15509 |
Source | Human GM-CSF R alpha, Fc Tag (orb545735) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 320 (Accession # P15509-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Loaded Human GM-CSF R alpha, Fc Tag on Protein A Biosensor, can bind Human GM-CSF, premium grade with an affinity constant of 9.21 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
Expression Region | Glu 23 - Gly 320 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_006131.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH7.5 |
Alternative names | GM-CSF R alpha,GM-CSF-R-alpha,GMCSFR-alpha,GMR-alp Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 60.9 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Loaded Human GM-CSF R alpha, Fc Tag (Cat. No. orb545735) on Protein A Biosensor, can bind Human GM-CSF, premium grade (Cat. No. orb257510) with an affinity constant of 9.21 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 60.6 kDa after removal of the signal peptide.The apparent molecular mass of GM-CSFR-hFc is approximately 70-130 kDa due to glycosylation. | |
Mammalian |