You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb545734 |
---|---|
Category | Proteins |
Description | Human GM-CSF R alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 320 (Accession # P15509-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 36.4 kDa |
Target | GM-CSF R alpha |
UniProt ID | P15509 |
Source | Human GM-CSF R alpha, His Tag (orb545734) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 320 (Accession # P15509-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Human GM-CSF R alpha, His Tag captured on CM5 Chip via anti-His antibody, can bind Human GM-CSF, premium grade with an affinity constant of 16.1 nM as determined in SPR assay (Biacore T200) (QC tested). |
Expression Region | Glu 23 - Gly 320 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_006131.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | GM-CSF R alpha,GM-CSF-R-alpha,GMCSFR-alpha,GMR-alp Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 36.4 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Human GM-CSF R alpha, His Tag (Cat. No. orb545734) captured on CM5 Chip via anti-His antibody, can bind Human GM-CSF, premium grade (Cat. No. orb257510) with an affinity constant of 16.1 nM as determined in SPR assay (Biacore T200) (QC tested).
98.00% | |
37.7 kDa (predicted). Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result. |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Recombinant Human GM-CSF R alpha Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Glu23-Gly320) of Human GM-CSF R alpha fused with His Tag and Avi tag at the C-terminal. |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Biotinylated Recombinant Human GM-CSF R alpha Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Glu23-Gly320) of Human GM-CSF R alpha fused with His Tag and Avi tag at the C-terminal. |
> 95% by SDS-PAGE. | |
Recombinant Human GM-CSF R alpha/CSF2RA Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Glu23-Gly320) of human GM-CSF R alpha/CSF2RA (Accession #P15509) fused with a 6��His Tag at the C-terminus. |