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Catalog Number | orb1807112 |
---|---|
Category | Assays and Kits |
Description | Human Glucagon Like Peptide 2 (GLP-2) ELISA Kit |
Reactivity | Human |
Range | 0.16-10ng/mL |
Assay Time | 1.5H |
Target | GLP2 |
Sensitivity | 0.1ng/mL |
Alternative names | GLP2 Read more... |
Note | For research use only |
Application notes | This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human GLP-2. During the reaction, Human GLP-2 in the sample or standard competes with a fixed amount of Human GLP-2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human GLP-2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human GLP-2 in tested samples can be calculated by comparing the OD of the samples to the standard curve. |
Sample Types | Serum, Plasma, Tissue homogenate and Other biological samples |
Expiration Date | Please enquire. |
Human | |
12.9 pg/mL-200 pg/mL | |
4.3 pg/mL |