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| Catalog Number | orb594873 |
|---|---|
| Category | Proteins |
| Description | Recombinant Human CD40 ligand (CD40LG), partial (Active) |
| Tag | Tag-Free |
| Form/Appearance | Lyophilized powder |
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 20 mM PB, 200 mM NaCl, 0.1 mM EDTA, pH 7.0 |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Protein Sequence | MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL |
| Protein Length | Partial |
| UniProt ID | P29965 |
| MW | 16.2 kDa |
| Application notes | Partial |
| Endotoxins | Less than 1.0 EU/µg as determined by LAL method. |
| Source | E.coli |
| Biological Origin | Homo sapiens (Human) |
| Biological Activity | Immobilized Human CD40-Fc at 5μg/ml (100 μl/well) can bind Human CD40L. The ED50 of Human CD40L is less than 10 ng/ml. |
| Expression Region | 113-261aa |
| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
| Alternative names | CD40 Ligand; CD40-L; T-Cell Antigen Gp39; TNF-Rela Read more... |
| Research Area | Immunology & Inflammation |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Greater than 87% as determined by SDS-PAGE. | |
44.5 kDa | |
Mammalian cell |
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Unconjugated | |
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. | |
Predicted: 42.7 KDa. Observed: 50&90 KDa, reducing conditions |
Biotin | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 20.9 KDa. Observed: 23-28 KDa, reducing conditions |
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