Human APP / Abeta42 Protein, His Tag

SKU: orb3158238

Description

Amyloid precursor protein (APP) is a type I integral membrane protein ubiquitously expressed in many tissues and concentrated in the synapses of neurons. It has three predominant splice variants: APP695, APP751, and APP770. The majority of APP is cleaved at the plasma membrane by the α-secretase in the non-amyloidogenic pathway. The amyloidogenic pathway starts with β-secretase cleavage by BACE1 on the N-terminal part of the Aβ domain, releasing sAPPβ from a membrane-anchored fragment named βCTF or C99, which is subsequently cleaved by γ-secretase to release Aβ.

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 6.3 kDa. The protein migrates as 8-9 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Key Properties

• Expression System: E. coli
• Target: APP
• Reactivity: Human
• Tag: C-10×His
• Molecular Weight: 6.3 kDa
• Expression Region: Asp 672 - Ala 713
• Purity: 95%
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C
• Form/Appearance: Powder
• Buffer/Preservatives: 20 mM NaOH
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

APP

RefSeq:P05067